A groundbreaking research initiative led by Prof. Jiang Changlong from the Hefei Institutes of Physical Science, part of the Chinese Academy of Sciences, has resulted in the development of a novel dual-mode sensing platform aimed at the rapid detection of neonatal jaundice. This innovative platform, highlighted in a research article published in Analytical Chemistry, integrates fluorescence and colorimetric methods to provide a highly sensitive and low-detection-limit solution for bilirubin detection in complex biological samples.

Importance of Early Jaundice Detection

Jaundice is a significant health concern among neonates, with approximately 60% of newborns affected by this condition, which can lead to early neonatal mortality if left undiagnosed. Early detection of elevated free bilirubin levels, which range from 1.7 μM to 10.2 μM in healthy individuals, is crucial—concentrations below 32 μM typically do not exhibit classic symptoms but can indicate potential health risks. Therefore, the ability to rapidly and accurately monitor bilirubin levels in neonates is essential.

Upconversion Nanoparticles: A Technological Innovation

The researchers noted that upconversion nanoparticles (UCNPs) exhibit considerable potential for detecting small molecules within biological samples due to their minimal background fluorescence interference. However, previous limitations inherent in UCNPs, such as low luminescence intensity, necessitated the need for enhanced probes. To progress beyond this constraint, the team implemented a zinc ion doping strategy which regulates the growth of upconversion nanocrystals, thereby improving the energy transfer efficiency of the nanoparticles and achieving heightened upconversion luminescence.

Key Findings

The study unveiled several significant advancements:

  • Development of a Near-Infrared Excited Platform: A 980 nm near-infrared excited upconversion visual sensing platform was created for serum bilirubin detection.
  • Innovative Nanoprobe Creation: By combining UCNPs with sulfosalicylic acid and iron ions, the researchers succeeded in forming a highly efficient nanoprobe, which produced observable changes in fluorescence and colorimetric gradient in the presence of bilirubin.
  • Portable Sensing Solution: Incorporating 3D-printing technology, the team constructed a portable sensing device that leverages a smartphone's color recognition function, facilitating rapid clinical testing for bilirubin monitoring.

Performance Metrics of the Sensing Platform

The effectiveness of the developed sensor was demonstrated, achieving a detection limit as low as 21.4 nM, enabling notably precise bilirubin detection amidst complex biological matrices. The findings underscore the sensor's strong upconversion luminescence emission, highlighting its substantial potential for early diagnosis of jaundice and related complications through sensitive biomarker detection.

Conclusion

In conclusion, this pioneering work represents a notable advancement in neonatal healthcare technology, providing clinicians with a robust tool for the early diagnosis and monitoring of jaundice. Given the prevalence of this condition among newborns, the implications of such a technology could significantly enhance patient outcomes. For more detailed insights into this study, refer to the original article.

References

[1] Zhang, L., et al. (2025). Zinc Doping-Induced Lattice Growth Regulation for Enhanced Upconversion Emission in Serum Bilirubin Detection. Analytical Chemistry.

[2] Prof. Jiang Changlong’s Research Work.